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KMID : 0903520050480040431
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 4 p.431 ~ p.434
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Quantitative Analysis of Antioxidants in Korean Pomegranate Husk (Granati pericarpium) Cultivated in Different Site
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Kwak Hye-Min
Jeong Hyun-Hee
Sohng Bang-Ho
Kim Jong-Kuk
Lee Jin-Man
Hur Jong-Moon
Song Kyung-Sik
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Abstract
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The quantitative analytical method for major antioxidants, ellagic acid and punicalagin, in pomegranate husk (Granati pericarpium) were established by HPLC. The optimal HPLC conditions were as follows: Column; Agilent Zorbax Eclipse XDB-C18 (4.6×150 mm, 5 μm), mobile phase; 1% formic acid in water (A) and 1% formic acid in MeCN (B) (gradient elution of 5% to 100% B for 50 min), flow rate; 0.8 ml/min., detection; UV 254 nm. The optimal pre-treatment conditions for HPLC analysis were as follows: 5 g of pomegranate husk in 100 ml of 95% EtOH, refluxed for 3 h. Under these analytical conditions, punicalagin and ellagic acid contents in Korean pomegranates husks which were cultivated in five different sites were determined. As results, the ellagic acid and punicalagin (as a mixture of α- and β-anomer) contents were the highest in Haepyung pomegranate husk (15.27 μg/mg) and Jangsung pomegranate husk (16.21 μg/mg), respectively.
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KEYWORD
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antioxidant, ellagic acid, punicalagin, quantitative analysis, pomegranate husk
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